A Function for the hnRNP A1/A2 Proteins in Transcription Elongation.

A Function for the hnRNP A1/A2 Proteins in Transcription Elongation.

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The hnRNP A1 and A2 proteins regulate processes such as alternative pre-mRNA splicing and mRNA stability.Here, we report that a reduction trophy husband apron in the levels of hnRNP A1 and A2 by RNA interference or their cytoplasmic retention by osmotic stress drastically increases the transcription of a reporter gene.Based on previous work, we propose that this effect may be linked to a decrease in the activity of the transcription elongation factor P-TEFb.Consistent with this hypothesis, the transcription of the reporter gene was stimulated when the catalytic component of P-TEFb, CDK9, was inhibited with DRB.

While low levels of A1/A2 stimulated the association of RNA polymerase II with the reporter gene, they also increased new balance 990 v3 red the association of CDK9 with the repressor 7SK RNA, and compromised the recovery of promoter-distal transcription on the Kitlg gene after the release of pausing.Transcriptome analysis revealed that more than 50% of the genes whose expression was affected by the siRNA-mediated depletion of A1/A2 were also affected by DRB.RNA polymerase II-chromatin immunoprecipitation assays on DRB-treated and A1/A2-depleted cells identified a common set of repressed genes displaying increased occupancy of polymerases at promoter-proximal locations, consistent with pausing.Overall, our results suggest that lowering the levels of hnRNP A1/A2 elicits defective transcription elongation on a fraction of P-TEFb-dependent genes, hence favoring the transcription of P-TEFb-independent genes.